Aug 14, 2016 12:27
7 yrs ago
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Russian term
Russian Federation
Native Russian speaker
Native in: Russian 
Works in: Russian to English, English to Russian
выравнивание по количеству анализируемой матричной РНК
Russian to English
Medical
Biology (-tech,-chem,micro-)
comparative CT method
Выравнивание по количеству анализируемой матричной РНК (мРНК) выполняется за счет амплификации гена домашнего хозяйства в сравниваемых образцах с последующим обсчетом результатов ΔΔCt методом, и использованием в качестве референсного образца, в котором уровень экспрессии целевых генов принимается за 100%, тРНК, выделенной из клеток Основного банка линии-продуцента.
Proposed translations
(English)
| 4 +2 | normalization to the amount of input mRNA |
Natalie
|
| 3 | Alignment/amplification based on the quantity of analyzed messenger RNA (mRNA) |
Erzsébet Czopyk
|
Proposed translations
+2
11 hrs
Selected
normalization to the amount of input mRNA
Normalization to the amount of input mRNA is performed by amplification of the housekeeping gene... etc.
Посмотрите объяснение вот здесь, например:
Normalization to input RNA implies starting with the same amount and quality of material in each sample.
http://www.biotechniques.com/protocols/PCR/Real-time_PCR/Nor...
Посмотрите объяснение вот здесь, например:
Normalization to input RNA implies starting with the same amount and quality of material in each sample.
http://www.biotechniques.com/protocols/PCR/Real-time_PCR/Nor...
4 KudoZ points awarded for this answer.
Comment: "Thank you very much! "
12 mins
Erzsébet Czopyk
Hungary
Hungary
Hungary
sworn translator, linguist, DPO
Native in: Hungarian (Variant: Hungary) 
Works in: Russian to Hungarian, Hungarian to Russian, Latvian to Russian, and 10 more.
Related KudoZ points
:
4
Alignment/amplification based on the quantity of analyzed messenger RNA (mRNA)
RNA-Seq - Wikipedia, the free encyclopedia
https://en.wikipedia.org/wiki/RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time. RNA-Seq is used to analyze the continually changing cellular transcriptome. ... The cellular RNA is selected based on the desired size range.
Linear mRNA amplification approach for RNAseq from limited amount ...
www.sciencedirect.com/science/article/pii/S0378111915003649
Initially, we performed RNAseq from two human mammary epithelial cell lines ... Poly (A)+ mRNA, polyadenylated messenger RNA;; RIN, RNA integrity number; ... The use of mRNA amplification for RNAseq using approaches based on PCR ... For analyzing transcript structure after library preparation, we sequenced at GS ...
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Note added at 14 mins (2016-08-14 12:42:33 GMT)
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A guide to the whole transcriptome and mRNA Sequencing ... - Exiqon
www.exiqon.com/.../Guidelines-NGS-whole-transcriptome-mRNA....
Number of reads, read length and single or paired end reads? ..... For mRNA Sequencing, poly-A RNA selection is performed using an Oligo-dT ... Mapping: Alignment of reads to the specified reference genome (Human, Mouse and Rat) ... Our data analysis pipeline is based on the Tuxedo software package, which in turn is ...
[PDF]Design and Analysis of Single-Cell Sequencing ... - Hubrecht Institute
www.hubrecht.eu/wp-content/uploads/2015/11/CELL2015.pdf
Single- cell analysis of a small number of genes can be performed with ... cell mRNA-sequencing methods with improved sensitivity and ... gle-cell sequencing technique is based on amplification of min- ..... In the past, amplified RNA from single cells was quantified with ...... Single-cell messenger RNA.
[PDF]An Introduction to Next-Generation Sequencing Technology - Illumina
www.illumina.com/content/.../illumina_sequencing_introducti...
Total RNA and mRNA Sequencing ... (CE) based sequencing instruments—the AB370 in 1987 and the AB3730xl in ... fragments are then PCR amplified and gel purified. ... Data Analysis—During data analysis and alignment, the newly identified ..... Researchers can perform high-quality de novo assembly using NGS reads ...
https://en.wikipedia.org/wiki/RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time. RNA-Seq is used to analyze the continually changing cellular transcriptome. ... The cellular RNA is selected based on the desired size range.
Linear mRNA amplification approach for RNAseq from limited amount ...
www.sciencedirect.com/science/article/pii/S0378111915003649
Initially, we performed RNAseq from two human mammary epithelial cell lines ... Poly (A)+ mRNA, polyadenylated messenger RNA;; RIN, RNA integrity number; ... The use of mRNA amplification for RNAseq using approaches based on PCR ... For analyzing transcript structure after library preparation, we sequenced at GS ...
--------------------------------------------------
Note added at 14 mins (2016-08-14 12:42:33 GMT)
--------------------------------------------------
A guide to the whole transcriptome and mRNA Sequencing ... - Exiqon
www.exiqon.com/.../Guidelines-NGS-whole-transcriptome-mRNA....
Number of reads, read length and single or paired end reads? ..... For mRNA Sequencing, poly-A RNA selection is performed using an Oligo-dT ... Mapping: Alignment of reads to the specified reference genome (Human, Mouse and Rat) ... Our data analysis pipeline is based on the Tuxedo software package, which in turn is ...
[PDF]Design and Analysis of Single-Cell Sequencing ... - Hubrecht Institute
www.hubrecht.eu/wp-content/uploads/2015/11/CELL2015.pdf
Single- cell analysis of a small number of genes can be performed with ... cell mRNA-sequencing methods with improved sensitivity and ... gle-cell sequencing technique is based on amplification of min- ..... In the past, amplified RNA from single cells was quantified with ...... Single-cell messenger RNA.
[PDF]An Introduction to Next-Generation Sequencing Technology - Illumina
www.illumina.com/content/.../illumina_sequencing_introducti...
Total RNA and mRNA Sequencing ... (CE) based sequencing instruments—the AB370 in 1987 and the AB3730xl in ... fragments are then PCR amplified and gel purified. ... Data Analysis—During data analysis and alignment, the newly identified ..... Researchers can perform high-quality de novo assembly using NGS reads ...
Note from asker:
| Isn't the main goal (of the procedure described in the sentence) to calculate the difference in expression between two samples using the comparative CT method? |
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