выравнивание по количеству анализируемой матричной РНК
English translation: normalization to the amount of input mRNA
| 12:27 Aug 14, 2016 |
| Russian to English translations [PRO] Medical - Biology (-tech,-chem,micro-) / comparative CT method | |||||||
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|  Selected response from: Natalie Poland Local time: 20:37 | ||||||
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| 4 +2 | normalization to the amount of input mRNA |
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| 3 | Alignment/amplification based on the quantity of analyzed messenger RNA (mRNA) |
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Answers
12 mins confidence: 
Alignment/amplification based on the quantity of analyzed messenger RNA (mRNA) Explanation: RNA-Seq - Wikipedia, the free encyclopedia https://en.wikipedia.org/wiki/RNA-Seq RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time. RNA-Seq is used to analyze the continually changing cellular transcriptome. ... The cellular RNA is selected based on the desired size range. Linear mRNA amplification approach for RNAseq from limited amount ... www.sciencedirect.com/science/article/pii/S0378111915003649 Initially, we performed RNAseq from two human mammary epithelial cell lines ... Poly (A)+ mRNA, polyadenylated messenger RNA;; RIN, RNA integrity number; ... The use of mRNA amplification for RNAseq using approaches based on PCR ... For analyzing transcript structure after library preparation, we sequenced at GS ... -------------------------------------------------- Note added at 14 mins (2016-08-14 12:42:33 GMT) -------------------------------------------------- A guide to the whole transcriptome and mRNA Sequencing ... - Exiqon www.exiqon.com/.../Guidelines-NGS-whole-transcriptome-mRNA.... Number of reads, read length and single or paired end reads? ..... For mRNA Sequencing, poly-A RNA selection is performed using an Oligo-dT ... Mapping: Alignment of reads to the specified reference genome (Human, Mouse and Rat) ... Our data analysis pipeline is based on the Tuxedo software package, which in turn is ... [PDF]Design and Analysis of Single-Cell Sequencing ... - Hubrecht Institute www.hubrecht.eu/wp-content/uploads/2015/11/CELL2015.pdf Single- cell analysis of a small number of genes can be performed with ... cell mRNA-sequencing methods with improved sensitivity and ... gle-cell sequencing technique is based on amplification of min- ..... In the past, amplified RNA from single cells was quantified with ...... Single-cell messenger RNA. [PDF]An Introduction to Next-Generation Sequencing Technology - Illumina www.illumina.com/content/.../illumina_sequencing_introducti... Total RNA and mRNA Sequencing ... (CE) based sequencing instruments—the AB370 in 1987 and the AB3730xl in ... fragments are then PCR amplified and gel purified. ... Data Analysis—During data analysis and alignment, the newly identified ..... Researchers can perform high-quality de novo assembly using NGS reads ... |
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11 hrs confidence: peer agreement (net): +2
peer agreement (net): +2normalization to the amount of input mRNA Explanation: Normalization to the amount of input mRNA is performed by amplification of the housekeeping gene... etc. Посмотрите объяснение вот здесь, например: Normalization to input RNA implies starting with the same amount and quality of material in each sample. http://www.biotechniques.com/protocols/PCR/Real-time_PCR/Nor... |
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