English to Russian Law/Patents Biology (-tech,-chem,micro-) Композиции на основе РНК-направляемых эндонуклеаз

In some embodiments, the secondary structure is formed by hybridizing all or part of the RNA segment to nucleotides in the 5' end of the protospacer-targeting sequence or segment, nucleotides in the middle of the protospacer-targeting sequence or segment, and/or nucleotides in the 3'- end of the protospacer-targeting sequence or segment.
In some embodiments, contiguous segments of the RNA segment hybridize to the protospacer-targeting sequence or segment.
In some embodiments, non-contiguous segment of the RNA segment hybridize to the protospacer-targeting sequence or segment.
In some embodiments, the secondary structure is a hairpin.
In some embodiments, the secondary structure is stable at room temperature or 37ºC.
In some embodiments, overall equilibrium free energy of the secondary structure is less than about 2 kcal/mol at a temperature between about 4ºC and about 50ºC, such as room temperature or 37ºC.

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