kerameos_ioanna (asker) Sep 3, 2009:

The instrument channels of the endoscopes were contaminated with the blood/virus mixture indicated in section 5 using a sterile injection syringe and stored for 60 minutes at room temperature. The endoscope was then placed into the machine listed under section 2 and prepared according to the programme sequence indicated in section 3. A total of 2 independent tests were carried out for the investigation.
After completion of the relevant test cycles, samples were taken using 5 swabs from the outer surface of the endoscope. In addition, 5 swab samples were taken after each test and control cycle in the bottom area of the machine internal spaces (sump).
The following points were selected as sampling points for the swabs on the endoscope:
• suction valve
• inlet of the instrument channel after detaching the coupling connection
• outlet at the distal end
• two swabs of the outer surface in the area of the control part and of the insertion tube


The swabs or sponges were shaken for 30 seconds (Vortex, 2500 rpm) immediately after preparation of the sample in 1 ml DMEM (Dulbecco's Mod, of Eagles Medium) and the remainder inoculated in portions of 50 ul each on the cell cultures of

Panagiotis Andrias (X) Sep 3, 2009:

. Μπορεις να δωσεις περισσοτερες πληροφοριες η συγκειμενο; Το υλικο που μπηκε στον Vortex τι ειχε απορροφησει πρωτα; Remainder θα μπορουσε να ειναι τοσο το remainder supernatant οσο και το remainder sediment. Τελικα τι προσεθεσαν στα κυτταρα, την υπολοιπη υπερκειμενη φαση η το υποκειμενο υγρο;