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Services
Translation, Editing/proofreading
Expertise
Specializes in:
Chemistry; Chem Sci/Eng
Biology (-tech,-chem,micro-)
Medical: Health Care
Medical: Pharmaceuticals
Medical (general)
Medical: Instruments
Rates
Portfolio
Sample translations submitted: 1
English to Swedish: Abstract: HIV induces production of IL-18 from intestinal epithelial cells that increases intestinal permeability and microbial translocation. General field: Medical Detailed field: Biology (-tech,-chem,micro-)
Source text - English Interleukin-18 (IL-18) is a pleiotropic cytokine of the IL-1 family with multiple context dependent functions. We and others have shown that HIV infection is accompanied by increased circulating levels of IL-18 along with decreased levels of its antagonist, Interleukin-18 Binding Protein (IL-18BP). The infection is also accompanied by intestinal inflammation and decreased intestinal integrity as measured by intestinal permeability, regeneration and repair. However, little is known concerning the relation between high level of IL-18 associated with the viral infection and intestinal permeability. Here we demonstrate that HIV treatment increases production of IL-18 and decreases that of IL-18BP production in human intestinal epithelial cell (IEC) lines. IL-18 causes apoptosis of the IEC by activating caspase-1 and caspase-3. It induces epithelial barrier hyperpermeability by decreasing and disrupting both tight and adherens junction proteins, occludin, claudin 2 and beta-catenin. Disorganization of F-actin was also observed in the IEC that were exposed to the cytokine. Moreover IL-18 decreases transepithelial electrical resistance (TEER) in Caco-2 and increases permeability in HT29 monolayers. The cells' treatment with IL-18 causes an increase in the expression of phosphorylated myosin II regulatory light-chain (p-MLC) and myosin light-chain kinase (MLCK), and a decrease in phosphorylated Signal Transducer and Activator of Transcription (p-STAT)-5. This increase in p-MLC is suppressed by a Rho-kinase (ROCK)-specific inhibitor. Interestingly, the levels of the cytokine correlate with those of LPS in the circulation in three different categories of HIV infected patients (HAART-naïve and HAART-treated HIV-infected individuals, and Elite controls) as well as in healthy controls. Collectively, these results suggest that the HIV-induced IL-18 plays a role in increased intestinal permeability and microbial translocation observed in HIV-infected individuals.
Translation - Swedish Interleukin-18 (IL-18) är ett pleiotrofiskt cytokin tillhörande IL-1 familjen med multipla miljöberoende funktioner. Vi liksom andra forskargrupper har visat att HIV infektion åtföljs av ökad cirkulerande nivå av IL-18 samt sänkta nivåer av dess antagonist ”Interleukin-18 Binding Protein” (IL-18BP). Infektionen åtföljs även av inflammation i tarmarna och sänkt tarmintegritet vilket mäts genom undersökning av tarmens genomsläpplighet, återbildande och reparation. Man vet dock väldigt lite om relationen mellan höga nivåer av IL-18, den virala infektionen och tarmpermeabiliteten och hur dessa faktorer är associerade till varandra. Vår studie visar att behandling mot HIV ger en ökad IL-18 produktion och sänkt IL-18BP produktion i humana cell-linjer från tarmepitel (Intestinal epithelial cell (IEC) lines). IL-18 orsakar celldöd hos IEC cell-linjer genom att aktivera caspase-1 och caspase-3. Det inducerar hyperpermeabilitet i tarmepitlets barriär genom att sänka nivån av och sönderdela de proteiner som kallas ”tight and adherens junction proteins” occludin, claudin 2 och beta-catenin, vilkas funkton är att vidhäfta cellerna vid varandra och skapa en icke permeabel barriär. Även disorganisation av F-actin observerades i det tarmepitel (IEC) som exponerades för cytokin. Vidare kunde en sänkning av transepitelialt elektriskt motstånd (TEER) uppmätas i närvaro av IL-18 hos epiteliala Caco-2 cell-linjer och en ökad genomsläpplighet i HT29 cell-”monolayers”. Behandling av cellerna med IL-18 orsakar ett ökat uttryck av foforylerat ”myosin II regulatory light-chain” (p-MLC) och ”myosin light-chain kinase” (MLCK), och en minskad nivå av fosforylerad ”Signal Transducer and Activator of Transcription” (p-STAT)-5. Denna ökning av p-MLC motverks av en Rho-kinase (ROCK)-specifik inhibitor. Nivåerna av cytokin korrelerar med LPS i cirkulationen i tre kategorier av HIV infekterade patienter (HAART-naïve and HAART-treated HIV-infected individuals, and Elite controls) liksom med nivåerna i den friska kontrollgruppen vilket är intressant. Sammantaget indikerar dessa resultat att närvaro av det HIV inducerade IL-18 proteinet är sammanlänkat med ökad tarmpermeabilitet och genomsläpplighet av mikrober genom tarmen som observerats hos HIV infekterade individer.
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Translation education
Other - Karolinska Institute
Experience
Years of experience: 6. Registered at ProZ.com: Mar 2018.
I am a protein biochemist and licensed biomedical
scientist that have been working with texts for many years within pharmacy,
laboratory medicine and science.
I got the following recommendation from a translator
with more than 25 years of experience. We worked together with a translation of
a huge manual for a heart lung machine.
"I can strongly recommend
her, she is a licensed medical scientist and biochemist. She knows how to write
correct Swedish"
Do you have a need of high quality translation that I
can help you with? Most welcome to contact me for questions. I willingly
provide a test translation. My aim is always to be available for my customers,
to do a very good job and to be honest and prepared to do changes in case it's
necessary.
I translate pharmaceutical information, drug package
leaflets, guides and instructor manuals for laboratory and medical equipment
and texts for books, articles and homepages within natural sciences.
For the moment I work for two customers which provides
different types of texts from the big pharma industry. I have for example
translated leaflets and documents for withdrawal of medical product. Since the
work I do is confidential I cannot show any sample of these translations.
However, I have translated a scientific abstract that can be viewed on this
site.
Yours Sincerely,
Ellinor Thidholm
Ph. Lic. in Cell and Molecular Biology, B. Sc. in
Chemistry, Licensed Biomedical Scientist
My CV can be
viewed on LinkedIn
Keywords: medicine, medical equipment, pharmaceuticals, chemsitry, biochemistry, cell and molecular biology, microbiology, drug information, package leaflets, biochemical kit manual. See more.medicine, medical equipment, pharmaceuticals, chemsitry, biochemistry, cell and molecular biology, microbiology, drug information, package leaflets, biochemical kit manual, laboratory equipment, natural science, scientific articles. See less.
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