метод почвенных комочков (метод обрастания комочков почвы)
English translation: soil grain technique
| 04:20 Jan 23, 2021 |
| Russian to English translations [PRO] Science - Biology (-tech,-chem,micro-) / микробиология, почвоведение | |||||||
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|  Selected response from: Vera Klink Canada Local time: 13:29 | ||||||
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| Summary of answers provided | ||||
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| 4 | method of soil samples [(1-2 mm diameter)]; (method of soil sampling)[(samples of diameter 1-2 mm)] |
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| 4 | Hagem's method |
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| 3 | soil grain technique |
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Answers
1 hr confidence: 
method of soil samples [(1-2 mm diameter)]; (method of soil sampling)[(samples of diameter 1-2 mm)] Explanation: This is the idiomatic manner in which the thought is Russian is expressed. There is no need to try to be more literal. -------------------------------------------------- Note added at 1 hr (2021-01-23 05:43:08 GMT) -------------------------------------------------- 1. )[( --> ) [(2 2. Reference (one among numerous similar examples readily retrieved): Processing LinkIsolation and Analysis of Microbial Communities in Soil ...www.ncbi.nlm.nih.gov › pmc › articles › PMC6126543 Jul 24, 2018 — Collection and Processing of Soil, Rhizosphere, and Root Field Samples. Excavation of plants. Label a wash pan and bucket with a sticky note ... by MR McPherson · 2018 · Cited by 23 · Related articles -------------------------------------------------- Note added at 1 hr (2021-01-23 05:45:27 GMT) -------------------------------------------------- 1. thought is Russian --> thought in Russian. 2. )[( --> ) [( 3. Reference (one among many similar references readlly retrieved): Processing LinkIsolation and Analysis of Microbial Communities in Soil ...www.ncbi.nlm.nih.gov › pmc › articles › PMC6126543 Jul 24, 2018 — Collection and Processing of Soil, Rhizosphere, and Root Field Samples. Excavation of plants. Label a wash pan and bucket with a sticky note ... by MR McPherson · 2018 · Cited by 23 · Related articles |
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3 hrs confidence:
Hagem's method Explanation: https://present5.com/metody-izucheniya-pochvennyx-gribov-ii-... Посев почвы без предварительной обработки • Метод почвенных комочков (Hagem, 1908, 1910) https://era.ed.ac.uk/handle/1842/30071 (там есть ссылка на pdf) An Investigation of the Mucorales in the Soil. By Marie E. Campbell In plating out the samples two methods were adopted, that of Hagem (1907) which consists of sprinkling a little of the soil over the surface of the Petri-dish containing nutrient medium. The second method is that described by Brierley (1927). A dilution of 1/20,000 in sterile water was made and then six poured plates were inoculated each with 1 c.c. of this soil suspension. As soon as the colonies began to show on the surface of the plates they were sub-cultured on to fresh media, thus giving pure multispore cultures. This precaution is necessary in order that the small forms may be obtained as well as the larger and stronger-growing forms https://www.jstor.org/stable/3757580?seq=1 Morphology and Nutrition of Chloridium chlamydosporis (Bisporomyces chlamydosporis) BS, Baarn. Stock cultures were maintained on beer-wort agar slants and stored at 12 C. They were initially inoculated from single spore cultures obtained by Hagem's method (18). Другие варианты перевода - чисто описательные. |
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1 day 2 hrs confidence:
soil grain technique Explanation: The method you describe sounds extremely similar to the method described by Aquilante et al (2004). There is no official name for it. The articles I've found referred to it as "soil grain technique," "soil grains-sowing technique," or similar. ---------------------------------------------------------------------------------- https://annalsmicrobiology.biomedcentral.com/articles/10.100... Azotobacter species were isolated using the soil grains-sowing technique as described previously (Aquilanti et al. 2004; Jiménez et al. 2011). Briefly, 30 g fresh soil was saturated with sterile water, and 3 g mannitol was added to the soil paste. The soil paste was then transferred and pressed into about 3 mm-diameter grains in a Petri dish using a sterile spatula. A total of 16 soil grains was placed on each Ashby-Sucrose agar plate (Jiménez et al. 2011), and each soil sample consisted of three replicates. The plates were incubated at 28°C for 7 days, after which sticky and glistening colonies around the grains were streaked and incubated on Ashby-Sucrose agar three times for purification. ----------------------------------------------------------------------------- http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517... The primary isolation was made using the grain soil technique (Figure 1A) according to the methodology previously described by Aquilanti et al. (1) and Becking (4) in Ashby-Sucrose agar (Agar 1.5%, Sucrose 0.5%, CaCO3 0.5%, MgSO4 0.02%, NaCl 0.02%, KH2PO4 0.02%, FeSO4 0.0005%). Plates were incubated at 28ºC for 7 days until observing sticky and glistening colonies around grains (Figure 1F). Counts were made for each cultivated soil (% Recovery = Number of grains with sticky and glistening colonies/total x 100). -------------------------------------------------------------------------------- The original technique is described here, if you can find the article: Aquilanti L, Favilli F, Clementi F (2004) Comparison of different strategies for isolation and preliminary identification of Azotobacter from soil samples. Soil Biol Biochem 36:1475–1483 |
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