буфер для невосстанавливаемых образцов
English translation: non-reducing buffer
| 06:19 Sep 6, 2016 |
| Russian to English translations [PRO] Medical - Biology (-tech,-chem,micro-) / процедура | |||||||
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|  Selected response from: Igor Andreev Local time: 07:16 | ||||||
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| 4 | buffer for non-reducible samples |
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| 3 +1 | non-reducing buffer |
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| 3 | non-recoverable sample buffer |
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Answers
1 hr confidence: 
non-recoverable sample buffer Explanation: N/A Reference: http://openwetware.org/wiki/SDS-PAGE_sample_buffer_(Morris_f... |
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3 hrs confidence:
buffer for non-reducible samples Explanation: non-reducing buffer - невосстанавливающий буфер non-recoverable sample buffer - буфер неизвлекаемых образцов Имеется в виду что они не способны к химическому восстановлению. Химическое восстановление - reduction. Так будет вернее чем non-reducing samples. |
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1 hr confidence: peer agreement (net): +1
peer agreement (net): +1non-reducing buffer Explanation: возможно имеются в виду невосстанавливающие условия, хотя недостаточно контекста, чтобы понять, что такое невосстанавливаемые образцы - non-reducible, irreducible or even non-recoverable ) Вопрос: их инкубируют в невосстанавливающих условиях или их попросту невозможно восстановить? -------------------------------------------------- Note added at 2 days13 hrs (2016-09-08 20:02:06 GMT) -------------------------------------------------- тогда возможно это buffer for non-reduced samples если это какой-то иной буфер для анализа на LabChip, отличный от non-reducing buffer, используемого для нагревания -------------------------------------------------- Note added at 2 days13 hrs (2016-09-08 20:04:12 GMT) -------------------------------------------------- т.е. буфер для невосстановленных образцов (тех, которые прогревали в невосстанавливающих условиях) -------------------------------------------------- Note added at 2 days13 hrs (2016-09-08 20:11:02 GMT) -------------------------------------------------- у "них" все проще описано :) Sample Preparation. 5 µL of each sample was combined with 35 µL sample buffer containing 0.7% LDS 1.9 mM Tris-Cl, 5.7% glycerol, and 1.7 mM EDTA. For reduced samples 24.5 µL of 1M DTT was added to 700 µL of sample buffer. For non-reduced samples 30 mM IAM was added to the sample buffer. http://www.level.com.tw/html/ezcatfiles/vipweb20/img/img/528... или For non-reducing conditions, 30mM IAM was added to the sample buffer as an alkylating agent. http://www.perkinelmer.co.jp/Portals/0/resource/products_ls/... |
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