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  3. Biology (-tech,-chem,micro-)

Контрастирование срезов

English translation: Contrast stain / counterstain / differential stain

23:58 Aug 4, 2016
Russian to English translations [PRO]
Medical - Biology (-tech,-chem,micro-) / immunohistochemistry
Russian term or phrase: Контрастирование срезов
Контрастирование срезов.
Проводили процедуру подкрашивания ядер гематоксилином в течение 10 мин., промывали дважды деионизированной водой.Обрабатывали срезы 1%-ным раствором HCl в течение 1 сек, промывали трижды деионизированной водой. Подсинивание ядер проводили в течение 45 сек. насыщенным раствором лития карбоната. После окончания окрашивания срезы промывали дважды деионизированной водой, дегидратировали в спиртовых растворах с повышающейся концентрацией спирта (70%, 80%, 96%), просветляли в заменителе ксилола, заключали в заливочную среду и монтировали покровные стекла с помощью прибора ClearVue (Thermo).
Artyom Timeyev
Russian Federation
Local time: 11:32
English translation:Contrast stain / counterstain / differential stain
Explanation:
Selected response from:

Maksym Nevzorov
Ukraine
Local time: 09:32
Grading comment
Thank you, Maksym!
4 KudoZ points were awarded for this answer



Summary of answers provided
4contrast of histology (tissue) sections
Marcombes (X)
4Differential staining of (histological) sections
Vera Klink
3Contrast stain / counterstain / differential stain
Maksym Nevzorov


Discussion entries: 1





  

Answers


6 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
Contrast stain / counterstain / differential stain


Explanation:


Maksym Nevzorov
Ukraine
Local time: 09:32
Works in field
Native speaker of: Native in RussianRussian
PRO pts in category: 44
Grading comment
Thank you, Maksym!
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11 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
contrast of histology (tissue) sections


Explanation:
-

Marcombes (X)
France
Local time: 08:32
Native speaker of: Native in FrenchFrench
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1 day 9 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
Differential staining of (histological) sections


Explanation:
Based on your discussion entry, the slides were stained with DAB and then hematoxylin? This would be called differential staining, which requires two or more dyes. In this case, DAB is the primary stain. Hematoxylin is used as a counterstain to DAB. The paragraph in your first post describes counterstaining with hematoxylin.

Basically, if both DAB and hematoxylin staining procedures are described under the same heading "Контрастирование срезов", then it's "Differential staining". If it's only for hematoxylin, then it's "Counterstaining".

Vera Klink
Canada
Local time: 00:32
Works in field
Native speaker of: Native in EnglishEnglish, Native in RussianRussian
PRO pts in category: 12
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