Restriktionsverdau
English translation: restriction digest
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| 13:46 Dec 14, 2006 |
| German to English translations [PRO] Science - Genetics | |||||||
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|  Selected response from: Steffen Walter Germany Local time: 21:13 | ||||||
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| 4 +4 | restriction digest |
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Answers
2 hrs confidence: 
peer agreement (net): +4
peer agreement (net): +4restriction digest Explanation: See parallel German/English sources http://www.biol.ethz.ch/IMB/groups/berger_group/Lectures/Pra... (see pp. 3, 4 - Table of Contents, Part B) http://www.botanik.univie.ac.at/lehre/lehrveranstaltungen/ss... and native English sources http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/dig... http://intranet.bpc.ac.uk/courses/download/bmsi/co/catering/... "Prepare a separate restriction digest for each presumptive positive PCR amplification, and amplification products from control Cyclospora cayetanensis and Eimeria tenella strains." http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/pr... "Stages in protocol (1) Restriction digest of PAC DNA (2) ligation of restriction digests (3) PCR amplification of ligation products (4) Sequencing of PCR products (5) Trim sequence of primer and vector sequence" http://ysp.ucdavis.edu/Research06/HuJ/default.html "Restriction digest: Specific restriction enzymes (Stu1, Pvu1, Dra1) were added along with their respective buffers to eluted PCR reactions and incubated at 37ºC for two to three hours. Afterwards, gel electrophoresis was conducted as with a regular PCR reaction." -------------------------------------------------- Note added at 2 hrs (2006-12-14 16:18:43 GMT) -------------------------------------------------- See also http://www.methodbook.net/dna/restrdig.html and http://preuss.bsd.uchicago.edu/protocols/enzyme.html - the longer version "restriction enzyme digest(ion)" is also used. |
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