détecteur (ELISA)

11:27 Sep 5, 2020
This question was closed without grading. Reason: No acceptable answer

French to English translations [PRO]
Medical - Biology (-tech,-chem,micro-) / UK English
French term or phrase: détecteur (ELISA)
Eviter la saturation du détecteur.

I can't give more context because this is one of a series of proposed answers in a multiple-choice question. In case it helps, here is the question: "A quoi sert la solution d'arrêt utilisée après coloration ?"

Detector? Sensor? Signal?
B D Finch
France
Local time: 13:13


Summary of answers provided
5context-sensitive: detector (detection agent/reagent) or sensor (instrument/device)
Johannes Gleim
1detection antibody
andres-larsen


Discussion entries: 2





  

Answers


2 hrs   confidence: Answerer confidence 1/5Answerer confidence 1/5
detection antibody


Explanation:
Enzyme Linked Immunosorbent Assay (ELISA) - StatPearls ...
www.ncbi.nlm.nih.gov › books › NBK555922
Mar 27, 2020 - Indirect ELISA requires two antibodies, a primary detection antibody that sticks to the protein of interest, and a secondary enzyme-linked antibody ...
M Alhajj - ‎2020 - ‎

Excerpt:

ELISAs are performed in polystyrene plates, typically in 96-well plates that are coated to bind protein very strongly. Depending on the ELISA type, testing requires a primary and/or secondary detection antibody, analyte/antigen, coating antibody/antigen, buffer, wash, and substrate/chromogen. The primary detection antibody is a specific antibody that only binds to the protein of interest, while a secondary detection antibody is a second enzyme-conjugated antibody that binds a primary antibody that is not enzyme-conjugated.

andres-larsen
Venezuela
Local time: 08:13
Does not meet criteria
Native speaker of: Native in EnglishEnglish
PRO pts in category: 4
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8 hrs   confidence: Answerer confidence 5/5
context-sensitive: detector (detection agent/reagent) or sensor (instrument/device)


Explanation:
Suzies link shows no "detector" or "sensor" but "detection antibody"
Sample:
After immobilization, a detection antibody is added, which binds to the adsorbed antigen thereby leading to the formation of an antigen-antibody complex. The detection antibody is either directly conjugated to an enzyme, such as horseradish peroxidase (HRP), or provides a binding site for a labeled secondary antibody.
https://www.bio-rad-antibodies.com/static/2017/an-introducti...

The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzə/, /ˌiːˈlaɪzə/) is a commonly used analytical biochemistry assay, first described by Engvall and Perlmann in 1971.[1] The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. … After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen.
:
A fourth ELISA test does not use the traditional wells. This test leaves the antigens suspended in the test fluid.[22][23]
1. Unlabeled antibody is incubated in the presence of its antigen (sample)
2. A sufficient incubation period is provided to allow the antibodies to bind to the antigens.
3. The sample is then passed through the Scavenger container. This can be a test tube or a specifically designed flow through channel. The surface of the Scavenger container or channel has “Scavenger Antigens” bound to it. These can be identical or sufficiently similar to the primary antigens that the free antibodies will bind.
4. The Scavenger container must have sufficient surface area and sufficient time to allow the Scavenger Antigens to bind to all the excess Antibodies introduced into the sample.
5. The sample, that now contains the tagged and bound antibodies, is passed through a detector. This device can be a flow cytometer or other device that illuminates the tags and registers the response.
https://en.wikipedia.org/wiki/ELISA

There are two main variations on this method: ELISA can be used to detect the presence of antigens that are recognized by a detection agent or it can be used to test for detection agents that recognize an antigen.
https://patents.google.com/patent/US7824867B2/en

Description | Quantity
Antibody Precoated Plate |1
Detection Antibody | 1
Detection Agent | 1
https://www.genorise.com/files/GSI_Equine_TNFa_ELISA_Kit-Uri...

This paper presents a compact and cost-effective lens-free complementary metal-oxide-semiconductor (CMOS) image sensor based bacterial cell detection platform that can measure colorimetric light signals in the enzyme linked immunosorbent assay (ELISA) process. In this work, we first demonstrate the CMOS image sensor based shadow imaging platform as an ELISA detector and provide critical performance data that show this platform to be comparable or even better than that of a commercial ELISA reader.
https://www.researchgate.net/publication/273654864_CMOS_imag...

matched pairs: capture (purified) and detection (biotin-conjugated) for human ELISA IL-4, Granzyme A, Granzyme B, sCD27
:
ELISA sets containing Capture, Detector and Standard enough for 5 microplates (480 tests)
http://www.immunotools.de/html/elisa.html
(note: the term "detector" is used for "detection"). Same below:

800™ TS Absorbance Reader
BioTek Instruments
• Wavelength(s): 340 to 750 nm
• Measurement Time: 11 seconds / 96 wells
• Optical System: Inquire
• Plate Formats: 6 to 384-well plates
• Detector: Inquire
https://www.biocompare.com/Lab-Equipment/20131-Microplate-Re...

Conventional enzyme immunosorbent assay (ELISA) has usually been performed in a centralized lab, requiring considerable time and sample/reagent consumption and expensive detection instruments.
https://www.researchgate.net/publication/303838455_Rapid_Det...

A CMOS Image SensorHaving Stacked Photodiodesfor Lensless ObservationSystem of Digital Enzyme-linked Immunosorbent Assay (ELISA)
:
The both photodiodes are monitored from N-type cathode while the both anodes are shorted to ground. In addition, the sensor in this work needs no bias modulation unlike the filter-less fluorescence detection sensor using photogate structure

Conclusion: the correct answer depends on the wider context. It’s either a detection device for measuring the colour intensity and/or turbidity/opacity or the agent used to detect the antigen (detection reagent).

Johannes Gleim
Local time: 13:13
Does not meet criteria
Native speaker of: German
PRO pts in category: 8
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