5X SSC

English translation: 5x concentared (0.75M NaCl, 0.075M) sodium chloride and sodium citrate solution

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:5X SSC
Selected answer:5x concentared (0.75M NaCl, 0.075M) sodium chloride and sodium citrate solution
Entered by: Veronica Prpic Uhing

17:23 Oct 31, 2005
English language (monolingual) [PRO]
Medical - Genetics
English term or phrase: 5X SSC
The probe was radiolabelled with a-32P-dCTP (3000 Ci/mmol, Amersham) by a random priming method. Hybridization was performed in 50% formamide, 5X SSC (0.75M NaCl, 0.075M sodium citrate), Denhardts solution (0.1% Ficoll, 0.1% polyvinylpyrrolidone, 0.l% BSA), and 0.l% SDS for 16 h at 42¡ãC.
envirostuden (X)
SSC" is sodium chloride and sodium citrate solution
Explanation:
SSC is a abbreviation of sodium chloride and sodium citrate solution

5X SSC (0.75M NaCl, 0.075M sodium citrate)

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Note added at 6 mins (2005-10-31 17:29:25 GMT)
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5x means it is 5 x concentrated and hence, needs to be diluted 5x in working buffer

SSC" is a abbreviation of sodium chloride and sodium citrate solution, which used to transfer nuclear acid from gel to membrane ( 20 *SSC or 10*SSC or 5*SSC with alkaline). In Roche's DIG system, they said that the high solt concentration SSC shows much efficiently than the others like valcuum or electrophoresis.
http://www.protocol-online.org/archive/posts/3129.html



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Note added at 58 mins (2005-10-31 18:21:36 GMT)
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Note added at 6 mins (2005-10-31 17:29:25 GMT)
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5x means it is 5 x concentrated and hence, needs to be diluted 5x into the working buffer


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Note added at 5 hrs 26 mins (2005-10-31 22:49:55 GMT)
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To XiaoRan: The real issue of importance here is the final concentrations of constituents in the assay. How you get there needs to be clarified ¨C 0.5 x of what, 2x of what, concentration of 5x (see above), especially when you decide to publish your data and communicate them to the others to understand and therefore better appreciate your work.

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Note added at 7 hrs 10 mins (2005-11-01 00:33:34 GMT)
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To XiaoRan: In this instance SSC is specified as: 5X SSC (0.75M NaCl, 0.075M sodium citrate),
Selected response from:

Veronica Prpic Uhing
United States
Local time: 06:04
Grading comment
Thanks!
4 KudoZ points were awarded for this answer



SUMMARY OF ALL EXPLANATIONS PROVIDED
5 +4SSC" is sodium chloride and sodium citrate solution
Veronica Prpic Uhing
5 +1five times of suggested working concentration of SSC
XiaoRan


Discussion entries: 4





  

Answers


10 mins   confidence: Answerer confidence 5/5 peer agreement (net): +1
5x ssc
five times of suggested working concentration of SSC


Explanation:
.

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Note added at 55 mins (2005-10-31 18:18:45 GMT)
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Yes, SSC is an abbreviation, no need to translate it at all.
Yes, what in parenthesis explain the word just before it.
I think it's easy to understand this, so I did not said it. Sorry.
(What I explained is the key word that may confuse people.)

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Note added at 6 hrs 58 mins (2005-11-01 00:21:47 GMT) Post-grading
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SSC is the abbreviation of "Solution of Sodium Citrate".

XiaoRan
Local time: 19:04
Specializes in field
Native speaker of: Native in ChineseChinese

Peer comments on this answer (and responses from the answerer)
agree  Jörgen Slet
4 hrs
Login to enter a peer comment (or grade)

4 mins   confidence: Answerer confidence 5/5 peer agreement (net): +4
5x ssc
SSC" is sodium chloride and sodium citrate solution


Explanation:
SSC is a abbreviation of sodium chloride and sodium citrate solution

5X SSC (0.75M NaCl, 0.075M sodium citrate)

--------------------------------------------------
Note added at 6 mins (2005-10-31 17:29:25 GMT)
--------------------------------------------------

5x means it is 5 x concentrated and hence, needs to be diluted 5x in working buffer

SSC" is a abbreviation of sodium chloride and sodium citrate solution, which used to transfer nuclear acid from gel to membrane ( 20 *SSC or 10*SSC or 5*SSC with alkaline). In Roche's DIG system, they said that the high solt concentration SSC shows much efficiently than the others like valcuum or electrophoresis.
http://www.protocol-online.org/archive/posts/3129.html



--------------------------------------------------
Note added at 58 mins (2005-10-31 18:21:36 GMT)
--------------------------------------------------

Note added at 6 mins (2005-10-31 17:29:25 GMT)
--------------------------------------------------

5x means it is 5 x concentrated and hence, needs to be diluted 5x into the working buffer


--------------------------------------------------
Note added at 5 hrs 26 mins (2005-10-31 22:49:55 GMT)
--------------------------------------------------

To XiaoRan: The real issue of importance here is the final concentrations of constituents in the assay. How you get there needs to be clarified ¨C 0.5 x of what, 2x of what, concentration of 5x (see above), especially when you decide to publish your data and communicate them to the others to understand and therefore better appreciate your work.

--------------------------------------------------
Note added at 7 hrs 10 mins (2005-11-01 00:33:34 GMT)
--------------------------------------------------

To XiaoRan: In this instance SSC is specified as: 5X SSC (0.75M NaCl, 0.075M sodium citrate),

Veronica Prpic Uhing
United States
Local time: 06:04
Native speaker of: Croatian
PRO pts in category: 4
Grading comment
Thanks!

Peer comments on this answer (and responses from the answerer)
agree  Ian M-H (X): saline sodium citrate, salt sodium citrate or standard saline citrate
3 mins
  -> Thank you Ian!

agree  Freimanis
31 mins
  -> Thank you!

neutral  XiaoRan: yes. the explanation of SSC is very good. But solutions such as 0.5 X buffer can also be used as working buffer. 5X solution can be diluted 5 times into a working buffer (1X), but it can also be diluted 10 times to make a working buffer (0.5X)
1 hr
  -> Theoretically yes, but in conventional and our lab 1X is the final concentration that you obtain from 5X in this instance, otherwise you would call initial concentrate 10X, 12X etc. Naturally, read your recipe carefully when using for the first time

agree  Drunya: Hmm... I thought SSC stands for Saline sodium citrate, basically making it sodium citrate. Never knew it has sodium chloride too
2 hrs
  ->  0.75M NaCl is not saline, that is why. Once 5x SSC is diluted to 1x then you get saline sodium citrate. Namely, saline (physiologic solution) is 0.15M NaCl, which is 300 milliosmolar that is isotonic

agree  Jörgen Slet
4 hrs
  -> Thank you!
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