loosely and nonspecifically bound antibodies

Spanish translation: anticuerpos unidos débilmente y de manera no específica

00:20 Aug 11, 2020

English to Spanish translations [PRO]
Medical - Medical: Instruments / loosely and nonspecifically bound antibodies

English term or phrase: loosely and nonspecifically bound antibodies

Buenos días,

Espero que puedan ayudarme con una expresión al español: “loosely and nonspecifically bound antibodies”, relacionada con un tampón de lavado. Muchas gracias:

“Binding of an antibody to its epitopes involves electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which create undesired background staining. To remove these loosely and nonspecifically bound antibodies, a thorough washing is required after each immunostaining step.”

GVL

Giovanni Castaneda KudoZ activity Questions: 2095 (10 open) (71 closed without grading) Answers: 0 Peru
Local time: 19:34

Spanish translation:anticuerpos unidos débilmente y de manera no específica

Explanation:
No utilizaría "enlace" y sí "unión" para traducir biding/bound aquí. La interesante discusión del enlace que aporta Patricia ( https://www.proz.com/kudoz/english-to-spanish/chemistry-chem... ) creo que merece la pena, aunque el caso en cuestión sea bien distinto a éste.

Diría así, por ejemplo,

La unión de un anticuerpo con sus determinantes antigénicos implica fuerzas electrostáticas e hidrofóbicas. Ciertos anticuerpos tienden a unirse de manera débil y no específica a determinantes antigénicos [ajenos], lo que genera una tinción de fondo no deseada. Para eliminar estos anticuerpos [que permanecen] unidos débilmente y de manera no específica, se lleva a cabo un lavado [intenso]

Selected response from:

Chema Nieto Castañón
Spain
Local time: 02:34

Grading comment

Muchas gracias, Chema.
4 KudoZ points were awarded for this answer

Summary of answers provided

4 +2

anticuerpos unidos débilmente y de manera no específica

Chema Nieto Castañón

Anticuerpos con enlaces débiles y no específicos

Patricia Fierro, M. Sc.

Answers

23 mins confidence:

Anticuerpos con enlaces débiles y no específicos

Explanation:
https://www.proz.com/kudoz/english-to-spanish/chemistry-chem...

Patricia Fierro, M. Sc.
Ecuador
Local time: 19:34
Native speaker of:

Spanish,

English
PRO pts in category: 32

1 hr confidence:

peer agreement (net): +2

anticuerpos unidos débilmente y de manera no específica

Chema Nieto Castañón
Spain
Local time: 02:34
Native speaker of:

Spanish
PRO pts in category: 348

Grading comment

Muchas gracias, Chema.

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loosely and nonspecifically bound antibodies

Spanish translation: anticuerpos unidos débilmente y de manera no específica

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loosely and nonspecifically bound antibodies

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